STUDIES ON INDIRECT THERMOOPTICAL DETECTI ON FOR CAPILLARY ELECTROPHORESIS

Indirect thermo-optical detection for capillary electrophoresis was pr esented first by combining indirect detection with thermo-optical meth od. In this detection, methylene blue solution was used as background electrolyte solution, because it has large molar absorption coefficien t at 633 nm. This wavelength matches well with that of He-Ne laser(632 .8 nm). The detection was applied to detect amino acids in capillary z one electrophoresis separation. The detection limit for lysine was 5X1 0(-6) mol/L (S/N=2).