THE APPLICATION OF AN NAD-SILICA BIOSPECI FIC AFFINITY-CHROMATOGRAPHYSTATIONARY-PHASE IN ANALYSIS OF NUCLEOTIDES

By using a wide-pore (30 nm), microspheric silica (8 mu m) as the matr ix and gamma-aminopropyltriethoxysilane as the coupling agent, a stati onary phase of biospecific affinity chromatography was prepared by che mical binding nicotinamide adenine dinucleotide (NAD) to the gamma-ami nopropyl modified silica, Using the phosphate buffer (PH = 7.0) as a m obile phase, the separation of some nucleotides is accomplished by the detection of UVD (260 nm). The effect of changed ion strengh of eluen t on the resolution of nucleotide (AMP, ADP and ATP) has been studied.