GEL CHROMATOGRAPHIC ANALYSIS OF CYCLOSPORINE AND ITS METABOLITES IN HUMAN BLOOD COMPARTMENTS

Gel chromatography combined with specific and non-specific cyclosporin radioimmunoassays was adopted for quantitative analysis of cyclospori n and metabolites in free and protein-bound forms in blood compartment s of kidney transplant patients. The analytical method was proved to b e useful for the purpose, although plasma protein-bound forms of neith er cyclosporin nor metabolites could be quantitated in the system. The present study also provided, by gel chromatographic analysis, additio nal examples to prove that concentrations of cyclosporin metabolites i n blood compartments may not be deduced or inferred simply from those of cyclosporin.