INFLUENCE OF VARIATION AT THE APOLIPOPROTEIN-E LOCUS ON LIPID AND LIPOPROTEIN LEVELS IN CAPD PATIENTS

Background, Variation at the apolipoprotein E (ape E) locus influence lipid and lipoprotein levels in the normal population, and is associat ed with premature coronary artery disease. Patients with end-stage kid ney disease or undergoing dialysis treatment are particularly prone to develop accelerated atherosclerosis. Methods. To evaluate the influen ce of genetic variation at the apo E locus, apo E genotypes and serum lipid and lipoprotein levels were measured in 51 subjects undergoing c ontinuous ambulatory peritoneal dialysis (CAPD). Results. The distribu tion of apo E phenotypes and apo E allelic frequency among the CAPD su bjects (epsilon 2 0.049; epsilon 3 0.745; epsilon 4 0.206) corresponde d to the healthy Swedish population. In the CAPD subjects, total serum and LDL cholesterol levels were high (6.7 +/- 1.5 mmol/l and 4.2 +/- 1.3 mmol/l respectively) and HDL cholesterol levels were low (1.2 +/- 0.5 mmol/l). When directly comparing the two major apo E groups, E 3/3 subjects (n = 30) and E4/3 and 4/4 subjects, epsilon 4-carriers, (n = 16), LDL cholesterol levels were significantly higher among E4-carrie rs (4.8 +/- 1.1 vs 4.0 +/- 1.2 mmol/l, P<0.03), but total serum choles terol levels was not higher among the E4-carriers (7.3+/-1.3 vs 6.5 +/ - 1.5 mmol/l, P < 0.08). Serum triglycerides or HDL cholesterol levels did not differ significantly between epsilon 3-homozygotes and epsilo n 4-carriers. Conclusions, The results demonstrate a strong effect of variation of the apo E locus on LDL cholesterol levels in CAPD subject s, suggesting that epsilon 4-carriers may be more susceptible to accel erated development of atherosclerosis in this condition.