METAL-CHARACTERIZATION OF N-ACYL-D-GLUTAMATE AMIDOHYDROLASE FROM PSEUDOMONAS SP STRAIN 5F-1

Authors
WAKAYAMA M MIURA Y OSHIMA K SAKAI K MORIGUCHI M
Citation
M. Wakayama et al., METAL-CHARACTERIZATION OF N-ACYL-D-GLUTAMATE AMIDOHYDROLASE FROM PSEUDOMONAS SP STRAIN 5F-1, Bioscience, biotechnology, and biochemistry, 59(8), 1995, pp. 1489-1492
Citations number
15
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
59
Issue
8
Year of publication
1995
Pages
1489 - 1492
Database
ISI
SICI code
0916-8451(1995)59:8<1489:MONAFP>2.0.ZU;2-A
Abstract
N-Acyl-D-glutamate amidohydrolase (D-AGase) from Pseudomonas sp. 5f-1 was a zinc-metalloenzyme which contained 2.06-2.61 g . atom of Zn per mole of enzyme. The zinc atom was required for the catalytic activity and stability of the enzyme. The N-terminal amino acid sequence of Pse udomonas sp. 5f-1 D-AGase showed 32% identity to that of Alcaligenes x ylosoxydans subsp. xylosoxydans A-6.