TRANSLATIONAL CONTROL OF ACTIVIN IN XENOPUS-LAEVIS EMBRYOS

Activin is a potent mesoderm inducing factor present in embryos of Xen opus laevis. Recent evidence has implicated activin in the inhibition of neural development in addition to the well-established induction of mesoderm in ectodermal explants. These diverse effects are critically dependent on the concentration of activin yet little is known about t he mechanisms regulating the level of activin in the embryo. We report that the 3' untranslated region (3' UTR) of activin PB mRNA inhibits the translation of activin in embryos. Micro-injection of activin mRNA from which the 3' UTR has been deleted is 8-10-fold more potent in in ducing mesoderm than mRNA containing the 3' UTR. Truncation of the 3' UTR also leads to a marked enhancement of activin protein levels in em bryos but has no effect when the truncated mRNA is translated in vitro . The 3' UTR also confers translational inhibition on a heterologous m RNA. These data show that a maternal factor(s) present in X. laevis re gulates the translation of injected activin PB mRNA. This factor(s) co uld be responsible for regulating the levels of endogenous activin PB protein during mesoderm induction and the specification of ectodermal derivatives such as neural and epidermal tissues. (C) 1995 Wiley-Liss, Inc.