GLUCAGON, STRESS, AND PORTAL-HYPERTENSION - PLASMA-GLUCAGON LEVELS AND PORTAL-HYPERTENSION IN RELATION TO ANESTHESIA AND SURGICAL STRESS

Glucagon has been proposed as the mediator of splanchnic hyperemia in portal hypertension. Employing an assay specific for pancreatic glucag on, we reevaluated the relationship between this peptide and portal hy pertension in the portal vein (PV)-stenosed rat model addressing, in p articular, the effects of anesthetic and surgical stress. Plasma gluca gon levels were similar in sham-operated and portal hypertensive rats: glucagon, sham vs PV stenosed: 110.7 +/- 17.1 pmol/liter vs 140.6 +/- 23.3 pmol/liter (NS). Furthermore, plasma levels of glucagon and the related peptide VIP were not significantly influenced by anesthesia or surgical stress, and levels remained similar under all conditions in sham-operated and PV-stenosed animals. We conclude that pancreatic glu cagon is not elevated in the PV-stenosed rat; differences between thes e results and those describing hyperglucagonemia in this model cannot be explained on the basis of a differential response to stress but may reflect differences in glucagon assay system.