SECRETORY MONOCLONAL IGA CLASS-SWITCH VARIANTS AGAINST BACTERIAL ENTERIC PATHOGENS IN BILE AND INTESTINAL SECRETIONS

In a previous study we analyzed the molecular forms of monoclonal IgA class-switch variants (moIgA variants) and their transport into murine respiratory secretions. The aim of the present study is to characteri ze the transport of moIgA variants into bile and intestinal secretions so that their applicability in a passive immunization model of the gu t can be evaluated. Different moIgA variants were directly isolated fr om IgG1 and IgG2a producing hybridoma clones specific for the same sur face determinants of bacterial enteric pathogens (Salmonella typhimuri um and Campylobacter jejuni) as their respective parent IgG clones. He patobiliary transport experiments clearly revealed the selective trans port of biologically active polymeric forms of the IgA variants into t he murine and rat bile after intravenous injection. Biotinylation of p olymeric IgA variants prior to intravenous injection resulted in the r ecovery of functional, labeled SIgA. Moreover biotin-labeled polymeric IgA variant was recovered in bile with an increased molecular weight, suggesting that the secretory component had been added during passage through the liver. When IgA variant and IgG parent clones were both u sed in a murine backpack tumor model for passive immunization, IgA var iant was selectively transported into intestinal secretions in compari son to IgG. The experimental model described here is suitable for use in comparative studies on the role of IgA and IgG with identical speci ficity in invasive infections of the intestinal tract.