IDENTIFICATION OF K1 K10 AND K5/K14 KERATIN PAIRS IN HUMAN-MELANOMA CELL-LINES/

Keratin expression in cultured malignant melanoma cells has been studi ed only rarely. Moreover, no studies have reported of universality of keratin expression in human malignant melanoma cells. In this study, t herefore, we analyzed keratin expression in eight cell lines. Using a low-salt aqueous solution without high salt and Triton X-100, as a was hing buffer for keratin extraction, followed by two-dimensional polyac rylamide gel electrophoresis (2D-PAGE) and immunological analysis, we demonstrated keratin expression in all eight human malignant melanoma cell lines. The keratin polypeptide expressions common to all melanoma cells were K1, K5, K10 and K14. In addition, K8, K13, K17 and K18, re spectively, were detected in individual cells. A measure of keratin ex pression universality in malignant melanoma cells may have implication s regarding their invasive and metastatic behaviors, co-expressed with vimentin.