J. Mcnair et al., DEVELOPMENT OF A SENSITIVE AND SPECIFIC TIME-RESOLVED FLUOROMETRIC IMMUNOASSAY FOR THE BOVINE ACUTE-PHASE PROTEIN HAPTOGLOBIN (HP), Journal of immunological methods, 184(2), 1995, pp. 199-205
Haptoglobin (Hp) is recognised as a major acute phase protein in the b
ovidae and its presence in serum is used as an indicator of inflammati
on. A mouse monoclonal antibody (1D9) specific for bovine Hp was label
led with a lanthanide (Eu) chelate and used to develop a competitive i
mmunoassay. This competitive immunoassay allowed direct measurement of
Hp in serum and was validated for intra- and interassay coefficients
of variation (below 8%). Cross-reactivity with other serum proteins wa
s measured (less than 0.1%) and limits of detection for Hp in serum we
re established for adult male (0.344 mu g/ml) and adult female cattle
(1.589 mu g/ml). The immunoassay was compared with an established hapt
oglobin-haemoglobin binding assay.