We have measured UV fluorescence excited through two-photon absorption
from native chicken tissue, using 600-nm, 500-fs pulses from a R6G dy
e laser. The observed emission signal was found to depend quadraticall
y on the excitation intensity. The two-photon excitation-induced fluor
escence spectrum is attributed to tryptophan residues in proteins. (C)
1997 Optical Society of America