EXPERIMENTAL IDENTIFICATION OF A SCALABLE REACTOR CONFIGURATION FOR LIGNIN PEROXIDASE PRODUCTION BY PHANEROCHAETE-CHRYSOSPORIUM

The present work concerns the experimental verification of a suitable scalable reactor configuration for lignin peroxidases production by Ph anerochaete chrysosporium. Tests were performed in 30 mi working volum e Erlenmayer flasks, 150 mi Fernbach flasks and a 5 cm ID, 83 cm high Trickle Fixed Bed Reactor working in batch mode with 250 mi of liquid medium. Shear stress, biofilm thickness and aeration have been shown t o be the key parameters for the control of lignin peroxidase productio n. Biofilm thickness was identified as a main scale up parameter. The results. compared with those found in the literature, are encouraging for further optimization of the reactor device and scale increase. Bio film thickness and gas and liquid superficial velocities have been ide ntified as variables in the optimization of lignin peroxidase producti on in a Trikle Fixed Bed Reactor configuration. Copyright (C) 1996 Els evier Science B.V.