MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF A SEED-EXPRESSED ACYL CARRIER PROTEIN (ACP) GENE FROM BRASSICA-CAMPESTRIS (AGRANI)

We describe here the nucleotide sequence of the Brassica campestris AC PSFI gene which encodes a seed-expressed acyl carrier protein (ACP). T he 3600 bp sequence consists of 1740 bp upstream of the translation st art codon, 828 bp spanning the coding region which is interrupted by t hree introns and 1032 bp downstream of the stop codon. Using a ACPSFI gene-specific probe, transcripts could be detected in developing seeds , but not in leaves. This gene is now the only known member that repre sents group I seed-expressed ACP multigene family of Brassica species. The 5' flanking sequence of the ACPSFI gene was examined for putative transcriptional regulatory elements. A sequence alignment of the 5' f lanking regions of the available seed-expressed ACP genes of Brassica species showed some conserved regions which might have some common reg ulatory significance.