ATTEMPT TO DISCRIMINATE BETWEEN BOVINE VIRAL-DIARRHEA VIRUS-STRAINS USING POLYMERASE CHAIN-REACTION

The polymerase chain reaction (PCR) was applied to detect bovine viral -diarrhoea mucosal-disease virus (BVDV). By the use of properly prepar ed primers, cytopathogenic NADL, Oregon C24V, Nose, T-20 and KS86-1(+) strains, and non-cytopathogenic New York-1, No. 12, and KS86-1(-) str ains could be detected. The PCR system was applied to field isolates o f the viruses. All the viruses were detected by the PCR. Four patterns of the PCR amplification were recognized, and it was possible to disc riminate between some strains. These results corresponded with the ser otype of BVDV, as determined by the serum-neutralizing test. The BVDV gene was detectable from the leucocytes of infected cattle using the P CR method. Moreover, it was possible to detect and discriminate BVDV s trains using one PCR tube that included all primer pairs.