COMPARISON OF KITS FOR THE DETERMINATION OF CREATINE-KINASE ACTIVITY IN SERUM

Thirteen kits from different suppliers for measurement of creatine kin ase activity in human serum according to the IFCC recommendations were analyzed and compared. Concentrations of AMP, ADP, creatine phosphate , glucose, magnesium ion, NADP(+), glucose-6-phosphate dehydrogenase, hexokinase and pH were measured in the reagents by various analytical techniques and compared with those recommended by the IFCC. We also co mpared by regression analysis the results of creatine kinase catalytic concentration obtained in human sera using commercial kits and in-hou se prepared reagents according to the IFCC recommendation. Creatine ki nase was also measured in a reference material using the different rea gents. The overall results of the activity measurements and the compos ition of the majority of the kits agree well with one another and with the IFCC recommendation. Minor deviations were found in the evaluatio n of a few kits. One kit yielded creatine kinase activity values that were 17% lower. Results obtained in the reference material measurement s showed differences with some kits which were not found using human s era.