IL-3-LA PRODUCTION BY MONONUCLEAR-CELLS OF PATIENTS WITH MULTIPLE-SCLEROSIS - EFFECT OF TREATMENT WITH INTRAVENOUS IMMUNOGLOBULINS

IL-3-like activity (IL-3-LA) is a growth factor that stimulates stem c ell maturation. We examined the production of IL-3-LA by peripheral bl ood mononuclear cells of 10 patients with relapsing-remitting multiple sclerosis (MS) following one year of treatment with intravenous immun oglobulins (IVIG). The results were compared with those obtained in 13 age- and sex-matched untreated patients with relapsing-remitting MS a nd in 14 healthy controls. IL-3-LA was assayed using the IL-3-dependen t 32-D-cl-23 murine cell line. IL-3-LA production was 60% higher in un treated MS patients than in healthy controls (134+/-19 u/ml and 78.7+/ -15.9 u/ml, respectively; p<0.01), and lower in patients treated with IVIG than in untreated patients (101.4+/-4.9 u/ml; p<0.02). IL-S-LA pr oduction also decreased after incubation of mononuclear cells with IVI G of both untreated MS patients and controls. This study indicates a p ossible beneficial effect of IVIG on the immunological status of MS pa tients.