M. Csabayova et al., RELATIVITY OF AN ANTIGENIC HOMOLOGY BETWEEN HUMAN INTERFERON-ALPHA-1 AND INTERFERON-ALPHA-2C, Immunological investigations, 24(5), 1995, pp. 787-793
Analysis of an antigenic relatedness between human interferon (IFN)-al
pha 1 and IFN-alpha 2 was performed with mapped monoclonal antibodies
raised to the respective subtypes. Antigenic properties of immunoreact
ive domains located in the N-terminal segments 30-67 of IFN-alpha 1 an
d IFN-alpha 2 were found distinct when compared by neutralization bioa
ssay or ELISA. On the other hand, corresponding domains exhibited an u
nexpectedly high antigenic homology when tested by Western blot. We su
ppose that this relativity in antigenic relation lies in the various e
xtent of denaturation of IFN-molecules in bioassay, ELISA and immunobl
ot. Structural differences of tested antigens may be responsible for a
conformation-determined access of antibodies to the shared epitopes.