TRANSFORMING GROWTH-FACTOR-BETA-2 IN LABIAL SALIVARY-GLANDS IN SJOGRENS-SYNDROME

Objective-To compare the distribution and the amount of transforming g rowth factor beta (TGF beta) in labial salivary glands (LSG) in patien ts with Sjogren's syndrome (SS) and healthy controls. Methods-LSG from SS patients (n = 10) and healthy controls (n = 6) were labelled with peroxidase-antiperoxidase staining for TGF beta 2, which was quantitat ed in image analysis using Video Interactive Display System software. Results-In all LSGs in SS and healthy controls, TGF beta 2 was found i n endothelial cells of the capillaries and in the capsular and stromal fibroblasts. In LSGs in SS, TGF beta 2 was also found in some lymphoc ytes in the inflammatory cell foci and in fibroblasts in fibrotic area s. The TGF beta 2 staining index (mu m(2)/mm(2) tissue) was greater in SS than in control LSGs (3670 (SEM 430) v 2061 (176); p < 0.01), with no difference between the primary and secondary forms of SS (p > 0.05 ). Conclusion-The localisation and the level of expression of TGF beta 2 indicate its involvement in local tissue fibrosis, and may reflect attempts at immunosuppression.