A NOVEL HUMAN MONOCLONAL-ANTIBODY AGAINST CERVICAL-CANCER - ITS IMMUNOREACTIVITY WITH NORMAL TUBE AND OVARY AND WITH OVARIAN TUMOR-TISSUE

1-1-2D, a novel human monoclonal antibody (MAb) raised against cervica l cancer, was examined for its immunohistochemical reactivity with ova rian cancer. Six of 10 ovarian cancer cell lines showed positive stain ing, while 3 of 5 cervical cancer cell lines were positive. Among tumo r tissues, 15 of 18 (83%) ovarian serous cystadenocarcinomas and 10 of 12 (83%) ovarian clear cell adenocarcinomas were positive. We also pe rformed immunohistochemical staining of the same cancer specimens with OC 125 and compared their reactivity. The frequency of positivity was similar, but the reactivity of the two MAbs was different. 1-1-2 D st ained the apical surface of the glandular epithelial cells and secreto ry products of the gland. On the other hand, OC 125 stained the cytopl asm as well as the plasma membrane of the glandular epithelial cells. These results suggest that 1-1-2 D MAb recognizes a different antigen from that recognized by OC 125.