Pregnant women and newborn infants were screened for group B streptoco
ccal (GBS) colonization by obtaining paired swabs from the cen ix and
urethra for the former group and from the ear, nose, umbilical cord, g
astric juice and membranes for the latter. One swab was cultured on bl
ood agar; the other was inoculated into serum-starch broth (GBS medium
), which allows identification of GBS by production of a characteristi
c orange-colored pigment. From the 2105 paired swabs obtained, a total
of 158 were GBS positive by either method; of these, 154 (97.5%) were
recovered by the GBS medium and 89 (56.3%) by blood agar plate. No fa
lse positive color reactions were observed with GBS medium. 75% of the
positive GBS media could be read within 24 h of incubation. The use o
f GBS medium proved to be an easy and reliable method for screening of
maternal and neonatal GBS colonization.