The crustacean isopod species Asellus aquaticus does not usually have
recognizable sex chromosomes. We previously identified a Y chromosome
marked by two heterochromatic bands in some males of a population from
the Sarno river near Naples. In this work we used oligonucleotide pro
bes to test the presence and possible sex-specific distribution of fiv
e simple repeat motifs in the genome of male and female individuals fr
om the Sarno population The five oligonucleotide probes were hybridize
d to enzyme-restricted genomic DNAs and the chromosome location of two
probes was tested using fluorescence in situ hybridization. Our resul
ts show that only the (TCC)(n) repetitive simple sequence has a sex-sp
ecific hybridization pattern and presents a significant accumulation o
n the Y chromosome in the region included between the two heterochroma
tic areas. Moreover the GGAAT sequence is not present in the genome of
A. aquaticus in any detectable quantity.