INDUCTION OF BENIGN PROSTATIC HYPERPLASIA IN INTACT DOGS BY NEAR-PHYSIOLOGICAL LEVELS OF 5-ALPHA-DIHYDROTESTOSTERONE AND 17-BETA-ESTRADIOL

Benign prostatic hyperplasia was induced in mongrel dogs treated for 6 0 days with one silastic implant containing 17 beta-estradiol and four containing 5 alpha-dihydrotestosterone. The condition was characteriz ed by (1) a marked increase of the stromal elements, particularly the stromal septa between the individual glands, (2) a slight increase in prostatic volume, and (3) a morphology that resembled spontaneous comp lex benign prostatic hyperplasia in the dog. Other groups of animals t hat remained untreated or received only 17 beta-estradiol or only 5 al pha-dihydrotestosterone did not develop this condition. Prostate volum es decreased by 14% in the estrogen-treated dogs, whereas they increas ed in the androgen-treated animals by 6% compared to pretreatment pros tate volumes. The morphology of the epithelium of the prostates of and rogen-treated animals was not different from that of controls despite the increase in prostate volume. The serum 17 beta-estradiol and 5 alp ha-dihydrotestosterone concentrations were increased from 25 +/- 2 (me an +/- SEM) and 256 +/- 42 pg/mL, respectively, in control dogs to 52 +/- 37 and 562 +/- 37 pg/mL, respectively, in the dogs treated with th e hormone combination. Thus, hormone concentrations were two- to three fold higher than control values, and the ratio of estradiol-17 beta to 5 alpha-dihydrotestosterone was increased by up to 19%. These data de monstrate that treatment of dogs with low levels of estrogen and andro gen may be an excellent model for the study of spontaneous complex ben ign prostatic hyperplasia in aging men. (C) 1995 Wiley-Liss, Inc.