EFFECTS OF CYCLOHEXIMIDE ON CHROMATIN-BOUND AND CHROMATIN-UNBOUND PCNA IN HELA-CELLS

Authors
SASAKI K KUROSE A UESUGI N KATO A
Citation
K. Sasaki et al., EFFECTS OF CYCLOHEXIMIDE ON CHROMATIN-BOUND AND CHROMATIN-UNBOUND PCNA IN HELA-CELLS, Oncology, 52(5), 1995, pp. 419-421
Citations number
13
Categorie Soggetti
Oncology
Journal title
Oncology → ACNP
ISSN journal
00302414
Volume
52
Issue
5
Year of publication
1995
Pages
419 - 421
Database
ISI
SICI code
0030-2414(1995)52:5<419:EOCOCA>2.0.ZU;2-2
Abstract
We examined the effects of cycloheximide on the amount of chromatin-bo und and -unbound PCNA subpopulations in HeLa cells. The chromatin-boun d PCNA content decreased rapidly in the presence of cycloheximide, whe reas the reduction of the total amount of PCNA was minimal even 24 h a fter the treatment with cycloheximide. From these data, the half-life of PCNA was estimated to be 31.0 h for chromatin-bound PCNA and 276.8 h for the total.