3-DIMENSIONAL OBSERVATION OF WHOLE-MOUNT CULTURED-CELLS STAINED WITH HISTOCHEMICAL REACTIONS BY ULTRAHIGH VOLTAGE ELECTRON-MICROSCOPY

Thick biological specimens prepared as whole mount cultured cells stai ned with histochemical reactions, such as thiamine pyrophosphatase, gl ucose-6-phosphatase, cytochrome oxidase, acid phosphatase, DAB reactio ns demonstrating specific cell organelles such as Golgi apparatus, end oplasmic reticulum, mitochondria, lysosomes, peroxisomes and pinocytot ic vesicles, were observed by ultrahigh voltage electron microscopy at accelerating voltages of 400-1000 kV producing stereo-pairs. As a res ult, those cell organelles were observed 3-dimensionally and the relat ive relationships between these organelles demonstrated.