Ppw. Vanbuul et al., MITOTIC AND MEIOTIC DETECTION OF RADIATION-INDUCED TRANSLOCATIONS IN MOUSE STEM-CELL SPERMATOGONIA USING FLUORESCENCE IN-SITU HYBRIDIZATION, Chromosome research, 3(7), 1995, pp. 427-432
The efficiency of two methods of detection of translocations induced i
n mouse stem cell spermatogonia by X-ray doses of 2, 5 and 7 Gy was co
mpared: classical multivalent analysis at diakinesis-metaphase I of me
iosis and observation via fluorescence in situ hybridization analysis
of mitotic or meiotic stages. Specific DNA libraries for chromosomes 1
, 11 and 13 were used. The results obtained indicate that (a) chromoso
mes 1, 11 and 13 are more involved in multivalent formation than expec
ted on the basis of DNA content and (b) if the mitotic FISH analysis d
ata are corrected for the observed over-representation, the frequencie
s of induced translocations are similar to those recorded in the class
ical multivalent studies, suggesting equal scoring efficiencies in bot
h systems.