Ma. Orsilles et al., TIME-COURSE OF REACTIVE OXYGEN INTERMEDIATES RELEASE AND HISTOPATHOLOGICAL FINDINGS DURING EXPERIMENTAL AUTOIMMUNE PROSTATITIS DEVELOPMENT, The Prostate, 27(1), 1995, pp. 50-57
Spontaneous and stimulated reactive oxygen intermediates (ROI) release
by peritoneal exudate cells (PEG) and histopathological findings in t
he prostate gland were assessed during experimental autoimmune prostat
itis (EAP) development. Results in EAP rats were compared with data fr
om rats immunized with kidney homogenate, BSA, and CFA, as well as non
treated rats. At 28 days of first immunization (FI), EAP rats spontane
ously released significantly more ROI than occurred in the cells from
controls rats. A similar response was found when ROI release was analy
zed after in vitro stimulus. In time course studies, an increased spon
taneous O-2(-) production was observed at day 7 after FI, and remained
the same during all period studied (14, 21, and 28 days after FI). Th
e stimulated O-2(-) production showed elevated levels at 7 days after
FI and fell afterward to levels similar to those of nontreated rats an
d increased again at day 28. Spontaneous or stimulated H2O2 release sh
owed a progressive increase during the study periods. ROI release was
correlated with infiltrate formation in the prostate gland. This diffe
rential responsiveness could indicate that, during the autoimmune proc
ess, the autoantigen(s) amplify the inflammatory response triggered by
them. (C) 1995 Wiley-Liss, Inc.