High pressure denaturation of ovalbumin, bovine serum albumin (BSA) an
d beta-lactoglobulin was assessed by spectrofluorometry, specific rota
tion analysis and differential scanning calorimetry (DSC), and compare
d with heat and chemical denaturation, lit all cases the denaturation
caused by high pressure was similar to that caused by the cleavage of
hydrogen bonds with urea or guanidine hydrochloride. (C) 1996 Academic
Press Limited