Ess. Couceiro et al., HEMAGGLUTINATING AND FUSOGENIC ACTIVITIES OF NEWCASTLE-DISEASE VIRUS - STUDIES ON RECEPTOR-BINDING SPECIFICITY AND PH-INDUCED CONFORMATIONAL-CHANGES, Memorias do Instituto Oswaldo Cruz, 90(4), 1995, pp. 515-520
Vaccinal and wild strains of Newcastle Disease virus (NDV) were analyz
ed for cell receptor binding and fusogenic biological properties assoc
iated with their HN (hemagglutinin-neuraminidase) and F (fusion protei
n) surface structures respectively. The evaluation of the biological a
ctivities of HN and F was carried out respectively by determination of
hemagglutinating titers and hemolysis percentages, using erythrocytes
from various animal origins at different pH values. Significant diffe
rences in hemagglutination titers for some strains of NDV were detecte
d, when interacting with goose, sheep, and guinea-pig and human ''O''
group erythrocytes at neutral pH. Diversity of hemolysis percentages w
as observed between different NDV strains at acid pH. These analysis w
ere developed to evaluate particular aspects of the actual influence o
f the receptor specificity and pH on the receptor binding and fusogeni
c processes of Newcastle Disease viruses.