SEPARATION OF ENANTIOMERS OF BENZODIAZEPINES ON THE CHIRAL-AGP COLUMN

The resolution of twenty-five 3-chiral and 5-chiral 1,4-benzodiazepine s and related compounds was studied on a Chiral-AGP column. Relationsh ip between the structure and enantioselective retention is discussed s tressing the role of hydrophobic and hydrogen-bonding interactions as well as the importance of the conformation of the enantiomers. The maj ority of the benzodiazepines were separated with high separation facto rs and high resolution. The enantioselectivity was influenced by the n ature and the concentration of the organic modifier in the mobile phas e, as well as by the pH. Chiral chromatographic separation was compare d with stereoselective binding on native AGP.