COMPLEX OF SIMIAN-VIRUS LARGE T-ANTIGEN AND P53 CAN BIND DNA SPECIFICALLY

The DNA-binding activity of p53 was suggested to be abrogated by bindi ng of large T antigen. However we demonstrate that a fraction of p53 c art form a complex with large T antigen and still bind DNA specificall y. In EMSA analysis, both PAb 416 and 419 anti-large-T antibodies coul d induce supershift on p53-DNA complex using extracts derived from two T-antigen-transformed cell lines. In EMSA-Western analysis, one of th e p53-complexes was shown to contain large T antigen.