SEQUENCE-ANALYSIS OF A 50 KB REGION BETWEEN SPOOH AND RRNH ON THE BACILLUS-SUBTILIS CHROMOSOME

The 49 630 bp spoOH-rrnH region of the Bacillus subtilis genome has be en fully sequenced. The sequence contains one partial and 62 complete ORFs, one partial and three complete rRNA genes and a cluster of six t RNA genes. The direction of the transcription and translation of 61 OR Fs is the same as that of the movement of the replication fork. A homo logy search of 40 ORFs in newly determined sequence revealed that 27 o f them had significant similarity to known proteins such as elongation factor G, elongation factor Tu, pseudouridine synthase I and ribosoma l proteins. Two adjacent genes, ybaD and ybaE, appeared to encode prot eins belonging to the ATP-binding cassette (ABC) family.