3 ATP1 GENES ARE PRESENT ON CHROMOSOME-II IN SACCHAROMYCES-CEREVISIAE

Chromosome fragmentation, ATP1 disruption, and Southern blot analyses of total DNAs and prime clones of chromosome II showed that three iden tical ATP1s are present, directing from the telomere to the centromere on the 35-55 kb far from the left telomere sequence of chromosome II. That is, the coding and 5'-, 3'-non-coding regions of ATP1 are repeat ed 3 times at approximately 7 kb intervals. These three ATP1s are expr essed, and one and two ATP1s-disrupted strains respectively, showed ca . 70 and 40% decreases in their ATPase activities and alpha subunit co ntents, compared to those of the wild-type, DC-5 or W303-1A strain, bu t could grow on glycerol.