M. Rose et Kd. Entian, NEW GENES IN THE 170-DEGREES REGION OF THE BACILLUS-SUBTILIS GENOME ENCODE DNA SUBUNITS, A THIOREDOXIN, A XYLANASE AND AN AMINO-ACID TRANSPORTER, Microbiology, 142, 1996, pp. 3097-3101
A DNA contig of 26.2 kb covering the 170 degrees region of the Bacillu
s subtilis strain 168 genome was isolated and sequenced. For DNA isola
tion, suitable restriction sites at the end of previously known genes
were chosen to amplify adjacent unknown DNA regions by inverse PCR. On
the basis of the DNA sequence, 26 ORFs were identified of which eglS
and ccdA, as well as parts of citB and tkt have been described previou
sly. Here we report the complete sequences of the aconitase (citB) and
transketolase (tkt) genes. Of the other proteins encoded on the 26.2
kb fragment, eight revealed similarities to previously described prote
ins. These included a pair of newly identified DNA gyrase subunits A (
grlA) and B (grlB), a sodium/proton-dependent alanine carrier (alsT),
a member of the thioredoxin family (tlpA), an endo-1.4-beta-xylanase (
xynD) and a response regulator protein. Comparison of the physical and
the genetic maps revealed several differences. According to its flank
ing sequences the lexA (dinR) gene which was previously mapped at 162
degrees was found to be adjacent to yneA localized at 170 degrees. Gen
es citB and eglS were located the opposite way round and closer togeth
er than expected from the genetic map (citB at 173 degrees and eglS at
170 degrees). The prkA gene, which was mapped at 169 degrees, was not
present on the respective fragment. Sequence comparison actually show
ed that prkA is located close to 70 degrees on the B. subtilis genome.