J. Cladera et al., INFLUENCE OF NUCLEOTIDES ON THE SECONDARY STRUCTURE AND ON THE THERMAL-STABILITY OF MITOCHONDRIAL F1 VISUALIZED BY INFRARED-SPECTROSCOPY, FEBS letters, 371(2), 1995, pp. 115-118
We have studied the secondary structure of mitochondrial F-1 using inf
rared spectroscopy, Our results show that in the absence of added nucl
eotides this complex contains similar percentages of alpha-helices, be
ta-structures and reverse turns (30%, 28% and 31%, respectively), The
influence of ADP and ATP on the different types of secondary structure
was determined; when all the nucleotide-binding sites were occupied,
small but reproducible changes were observed, corresponding to a decre
ase in beta-structure and an increase in alpha-helix and reverse turns
. The effect of nucleotide binding on the thermal stability of F-1 was
also studied; the thermal denaturation temperature, 55 degrees C, was
increased by 11 degrees C and 7 degrees C by ATP and ADP, respectivel
y. These results indicate that nucleotide binding affects the secondar
y structure of F-1, stabilizing the complex.