SIMULTANEOUS MEASUREMENT OF THE RATES OF APPEARANCE OF PALMITIC AND LINOLEIC-ACID IN CRITICALLY ILL INFANTS

Lipolysis has been measured in humans by means of stable isotope techn iques using labeled palmitic acid (PA) or glycerol as tracers. If othe r fatty acids (FA) such as linoleic acid (LLA) have the same rate of a ppearance (R(a)) as PA and therefore contribute equally to oxidative a nd nonoxidative metabolism is unknown. We infused albumin-bound [U-C-1 3]PA and [U-C-13]LLA in seven critically ill infants (weight 3.6 +/- 1 .3 kg, age 57 +/- 64 d) receiving 20.9 +/- 5.4 kcal . kg(-1). d(-1) of i.v. glucose only, and measured simultaneously the R(a) of PA and LLA from the isotopic enrichment of plasma FFA by mass spectrometry. A ne edle biopsy of the s.c. adipose tissue was obtained for FA composition . PA in adipose tissue was higher than LLA (40 +/- 6.7 versus 5.4 +/- 3.2 mol %, p < 0.001). The R(a) values of PA and LLA were 5.73 +/- 2.7 9 and 1.34 +/- 0.92 mu mol . kg(-1). min(-1), respectively (p = 0.005) . However, the ratio of the FA's R(a) to their respective mol% values in adipose tissue was lower for PA than for LLA (0.15 +/- 0.06 versus 0.25 +/- 0.06, p = 0.02). The R(a) of LLA acid was higher than could b e expected from the FA composition of adipose tissue, thus indicating a preferential release of LLA during lipolysis. In critically ill infa nts receiving only i.v. glucose, the contribution of LLA to the oxidat ive and nonoxidative metabolism may be larger than what assumed from t he FA composition of plasma and adipose tissue.