J. Heilmann et al., INDUCTION OF DNA DOUBLE-STRAND BREAKS IN CHO-K1 CELLS BY CARBON-IONS, International journal of radiation biology, 68(2), 1995, pp. 153-162
Citations number
51
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO
-K1 cells by means of an experimental approach involving constant-fiel
d gel electrophoresis and densitometric scanning of ethidium bromide s
tained gels. For X-irradiation, an induction efficiency of 36 +/- 5 ds
bs (Gy X cell)(-1) was determined. With this set-up, the induction of
dsbs was investigated in CHO-K1 cells after irradiation with accelerat
ed carbon ions with specific energies ranging from 2.7 to 261 MeV/u. T
his set of particle beams covers the important linear energy transfer
(LET) range between 17 and 400 keV/mu m, where maximum efficiencies ha
ve been reported for other cellular endpoints like inactivation or mut
ation induction. For LETs up to 100 keV/mu m, RBEs of similar to 1 hav
e been determined, while efficiencies per unit dose decline for higher
LETs. No RBE maximum > 1 was found. Data are compared with published
results on dsb induction in mammalian cells by radiations of comparabl
e LET.