DETECTION OF DNA POLYMORPHISM AMONG PEA CULTIVARS USING RAPD TECHNIQUE

An influence of some Random Amplified Polymorphic DNA (RAPD) reaction factors on resulting banding pattern and the ability of RAPD technique to detect DNA polymorphism among six economically important pea culti vars was tested. Relatively high level of DNA polymorphism among peas was observed, using polyacrylamide/urea gels and silver staining. Alto gether 13 arbitrarily designed primers produced 313 amplification prod ucts. In addition 59 polymorphisms were found. These polymorphisms can serve as potential genetic markers. RAPD data were processed using cl uster analysis and plotted as dendrogram. Each tested cultivar was cle arly distinguished from the others. Moreover, Pisum sativum and P. sat ivum subsp. arvense cultivars were separated into 2 different clusters , according to their systematic relationships.