IMPROVEMENT OF ARABIDOPSIS-THALIANA SEED TRANSFORMATION EFFICIENCY

Citation
D. Pavingerova et M. Ondrej, IMPROVEMENT OF ARABIDOPSIS-THALIANA SEED TRANSFORMATION EFFICIENCY, Biologia plantarum, 37(3), 1995, pp. 467-471
Citations number
NO
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00063134
Volume
37
Issue
3
Year of publication
1995
Pages
467 - 471
Database
ISI
SICI code
0006-3134(1995)37:3<467:IOASTE>2.0.ZU;2-2
Abstract
Agrobacterium tumefaciens induced transgenosis by treatment of germina ting Arabidopsis thaliana seed embryos has been achieved with differen t Agrobacterium strains including the strain LBA4404, which was ineffe ctive in seed transformation experiments of the other authors. The fre quency of transgenosis was increased several times by application of a cetosyringone to the growing A. tumefaciens suspension cultures. The D NA demethylating agent 5-azacytidine partly restored the distorted Men delian segregation ratios in the offspring of transgenic plants.