IMPROVEMENT OF ARABIDOPSIS-THALIANA SEED TRANSFORMATION EFFICIENCY

Agrobacterium tumefaciens induced transgenosis by treatment of germina ting Arabidopsis thaliana seed embryos has been achieved with differen t Agrobacterium strains including the strain LBA4404, which was ineffe ctive in seed transformation experiments of the other authors. The fre quency of transgenosis was increased several times by application of a cetosyringone to the growing A. tumefaciens suspension cultures. The D NA demethylating agent 5-azacytidine partly restored the distorted Men delian segregation ratios in the offspring of transgenic plants.