COLORIMETRIC MICROTITER PLATE HYBRIDIZATION ASSAY USING MONOCLONAL-ANTIBODY FOR DETECTION OF AN AMPLIFIED HUMAN-IMMUNODEFICIENCY-VIRUS TARGET

Since detection of amplified polymerase chain reaction products is a c omplicated procedure, a colorimetric microtiter plate hybridization as say was developed to automate specific detection of amplified HIV targ ets. In this assay, hybridization is seen by an antibody reacting sele ctively with double-stranded DNA. One hundred and ten amplified produc ts detected by a DNA enzyme immunoassay (DEIA) and by classical hybrid ization with a digoxigenin-labeling probe, detection sensitivities wer e less than 10 HIV targets per 10(6) cells. This study demonstrates th e specificity and sensitivity of DEIA for detecting amplified HIV targ ets. The use of the same equipment as for ELISA and the complete autom ation of the procedure allow a large number of samples to be processed in the clinical laboratory.