SEQUENCE-ANALYSIS OF A 44 KB DNA FRAGMENT OF YEAST CHROMOSOME-XV INCLUDING THE TY1-H3 RETROTRANSPOSON, THE SUFL(-RNA-GLY, THE YEAST TRANSFER RNA-THR-1A AND A DELTA-ELEMENT() FRAMESHIFT SUPPRESSOR GENE FOR TRANSFER)

We have sequenced on both strands a 44,019 bp fragment located on the left arm of Saccharomyces cerevisiae chromosome XV. The sequenced segm ent contains 22 open reading frames (ORFs) of at least 100 amino acids long, one of which probably contains an intron. Six of the 22 ORFs co rrespond to known proteins: the multicopy suppressor of Snf1 protein 1 , the two Tyl-H3 transposon proteins TyA and TyB, the myo-inositol tra nsporter 2, the transcription factor protein Ino4 and the 3,4-dihydrox y-5-hexaprenylbenzoate methyltransferase. Of the 16 remaining ORFs, tw o show highest homologies with the yeast serine/threonine protein kina se Ste20 and the human tryptophanyl-tRNA synthetase. Eight ORFs show s light similarities with protein sequences described in data banks. DNA sequence comparison reveals also the presence of three known sequence s: the Tyl-H3 transposable element, the yeast suf1(+) frameshift suppr essor gene for tRNA-Gly and the yeast transfer RNA-Thr-la. A fourth DN A sequence shows striking identities with the yeast delta elements. Th e 44,019 bp sequence has been entered in the EMBL data library under A ccession Number Z48149.