SEQUENCING ANALYSIS OF A 24.7 KB FRAGMENT OF YEAST CHROMOSOME-XIV IDENTIFIES 6 KNOWN GENES, A NEW MEMBER OF THE HEXOSE TRANSPORTER FAMILY AND 10 NEW OPEN READING FRAMES

The DNA sequence of a 24.7 kb region covering the left arm of chromoso me XIV from Saccharomyces cerevisiae was determined. This region conta ins 17 open reading frames (ORFs) which code for proteins of more than 100 amino acids. Five ORFs correspond to the KRE1, ATP11, DAL82, RFA2 and MCK1 loci, described previously. Two ORFs present high similarity to known proteins: NO345 with the hexose transporter family, and NO35 1 with the yeast chorismate mutase/prephenate dehydratase enzyme encod ed by PHA2. Six ORFs show limited similarity with known proteins or so me specific features: NO339 presents 11 potential transmembrane domain s. NO343, which is internal to NO345, presents a putative signal seque nce and a potential transmembrane domain. NO348 shows similarity with YCW2, TUP1 and SEC13. NO364 reveals a signature for a pyridoxal-phosph ate attachment site. Finally, NO384 and NO388 present a biased amino a cid composition, being rich in Asn or Glu/Lys/Arg, respectively. Four other ORFs (NO342, NO376, NO381 and NO397) show no similarity to prote ins within the databases screened. The sequence has been entered in th e EMBL data library under Accession Number Z46259.