A NEW ESSENTIAL GENE GCR4 LOCATED IN THE UPSTREAM REGION OF GCR1

Authors
UEMURA H JIGAMI Y
Citation
H. Uemura et Y. Jigami, A NEW ESSENTIAL GENE GCR4 LOCATED IN THE UPSTREAM REGION OF GCR1, Yeast, 11(11), 1995, pp. 1093-1101
Citations number
29
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology",Biology
Journal title
Yeast → ACNP
ISSN journal
0749503X
Volume
11
Issue
11
Year of publication
1995
Pages
1093 - 1101
Database
ISI
SICI code
0749-503X(1995)11:11<1093:ANEGGL>2.0.ZU;2-B
Abstract
A new essential gene of Saccharomyces cerevisiae was found upstream of GCR1. Its cloning and sequencing predict a 280 amino acid protein (32 577 Da). The predicted protein is fairly hydrophobic, and a search of the database did not identify any homologous proteins. A LEU2 disrupt ion at codon 104 was lethal, but disruption at codon 221 showed a temp erature-sensitive conditional growth phenotype. Abnormalities were obs erved in some glycolytic enzyme levels. The sequence has been submitte d to GenBank-EMBL-DDBJ under Accession Number D29645.