SEQUENCE-ANALYSIS OF A 78-CENTER-DOT-6 KB SEGMENT OF THE LEFT END OF SACCHAROMYCES-CEREVISIAE CHROMOSOME-II

We report the sequence analysis of a 78,601 bp DNA segment on the left arm of chromosome II of Saccharomyces cerevisiae. This 78.6 kb segmen t spans the region from the start of a subtelomeric Y' element up to t he ILS1 gene. It contains 49 open reading frames (ORFs) with more than 100 amino acids length including 14 internal and five overlapping ORF s. The gene density, excluding the internal ORFs, was calculated as on e ORF per 2.2 kb. Eight ORFs (PKC1, TyA, TyB, ATP1, ROX3, RPL17a, PET1 12 and ILS1) correspond to previously characterized genes. ORF YBL0718 was identified as CDC27; YBL0706 as TEL1. Four other ORFs show strong similarities to already known genes. The gene product of YBL0838 is 6 0% identical to the ribosomal protein RPL32 from rat, mouse and man. Y BL0701 encodes a protein with significant similarity to the initiation factor eIF2 associated p67 glycoprotein from rat. Eight ORFs were dis rupted and the resulting yeast strains analysed with respect to their phenotype. The sequence has been deposited in the EMBL Nucleotide Sequ ence Database under the Accession Number X79489.