EXTRACELLULAR PROTEASES OF HENDERSONULA-TORULOIDEA SCYTALIDIUM-HYALINUM AND SCYTALIDIUM-JAPONICUM

Citation
Ca. Oyeka et Hc. Gugnani, EXTRACELLULAR PROTEASES OF HENDERSONULA-TORULOIDEA SCYTALIDIUM-HYALINUM AND SCYTALIDIUM-JAPONICUM, Mycopathologia, 130(2), 1995, pp. 67-70
Citations number
22
Categorie Soggetti
Mycology,Pathology
Journal title
ISSN journal
0301486X
Volume
130
Issue
2
Year of publication
1995
Pages
67 - 70
Database
ISI
SICI code
0301-486X(1995)130:2<67:EPOHS>2.0.ZU;2-C
Abstract
Proteolytic activity was demonstrated in all the six clinical isolates of Hendersonula toruloidea, five of Scytalidium hyalinum and one of S . japonicum, screened, by using bovine serum albumin as the substrate. Assay of crude enzyme filtrates showed that enzyme activity increased with the age of the organism reaching a peak after twelve days of gro wth. The optimum temperature and pH for maximal enzyme activity were 3 7 degrees C and 5.6 respectively. The maximum dry weight of mycelia re corded were H. toruloidea, 135 mg; S. hyalinum, 100 mg; and S.japonicu m 103 mg/50 ml broth after 22 days of growth. Proteases may play a rol e in the pathogensis of infection caused by these fungi by hydrolyzing keratinized tissues such as stratum corneum and nails of humans.