S. Bhaskaran et Rh. Smith, EFFECT OF PROTEOLYTIC AND GLYCOLYTIC-ENZYMES ON A FACTOR IN SORGHUM-BICOLOR THAT INDUCES MYCELIAL GROWTH IN THE SMUT FUNGUS, SPORISORIUM-REILIANUM, Mycopathologia, 130(2), 1995, pp. 95-101
Proteins obtained from seedling shoots and floral meristems of Sorghum
bicolor (L.) Moench cv. NK 1210 induced mycelial growth in the smut f
ungus, Sporisorium reilianum in vitro. Proteins precipitated with tric
hloroacetic acid and ammonium sulfate were equally effective as induce
rs, although there were minor variations in the pattern of mycelial gr
owth. Hydrolysis of the protein fraction with the proteolytic enzyme p
ronase E resulted in considerable reduction in the proteins' ability t
o induce mycelial growth. Digestion of the protein fraction with drise
lase, resulted in a slight enhancement of biological activity. The res
ults suggest that amino sugar moieties in glycoproteins may act as ind
ucers of mycelial growth in Sporisorium reilianum.