CLONING AND RESTRICTION-ENDONUCLEASE MAPPING OF HERPES-SIMPLEX VIRUS TYPE-1 STRAINS H129 AND +GC

EcoRI fragments of herpes simplex virus I (HSV-1) strains H129 and +GC were cloned and the EcoRI and BglII restriction enzyme sites were map ped. Comparison of these enzyme sites with the sequence of HSV-1 strai n 17syn+ demonstrated that all EcoRI sites were identical. For H129, t he BglII sites were also found to match strain 17syn+ BglII sites. Wit h one exception, the BglII sites in strain +GC also aligned with the s train 17syn+ sequence. The one exception was a missing BglII site from strain +GC located between bases 25 149 and 25154 in the EcoRI D frag ment within the viral deoxyribonuclease gene (UL(12)). The BglII site represents the first difference to be mapped within HSV-1 strains 1112 9 and +GC which have unique pathobiological properties in animal model s of acute and reactivated infections.