Rotaviruses from environmental samples have been genotyped by a semine
sted reverse transcription PCR assay with serotype-specific primers de
rived from variable regions of gene 9, which produce different charact
eristic segment sizes for serotypes 1 to 4. The method enabled the det
ection and identification of type 1, 2, and 3 group A rotaviruses in s
ewage.