NESTED PCR METHOD FOR RAPID AND SENSITIVE DETECTION OF VIBRIO-VULNIFICUS IN FISH, SEDIMENTS, AND WATER

Authors
ARIAS CR GARAY E AZNAR R
Citation
Cr. Arias et al., NESTED PCR METHOD FOR RAPID AND SENSITIVE DETECTION OF VIBRIO-VULNIFICUS IN FISH, SEDIMENTS, AND WATER, Applied and environmental microbiology, 61(9), 1995, pp. 3476-3478
Citations number
18
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
9
Year of publication
1995
Pages
3476 - 3478
Database
ISI
SICI code
0099-2240(1995)61:9<3476:NPMFRA>2.0.ZU;2-6
Abstract
A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal pri mers flanking the V. vulnificus-specific sequences directed against 23 S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 c ells in artificially inoculated samples without enrichment and within 24 h.