MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF IMMOBILIZED DUPLEX DNA PROBES/

Authors
TANG K FU DJ KOTTER S COTTER RJ CANTOR CR KOSTER H
Citation
K. Tang et al., MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF IMMOBILIZED DUPLEX DNA PROBES/, Nucleic acids research, 23(16), 1995, pp. 3126-3131
Citations number
25
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
23
Issue
16
Year of publication
1995
Pages
3126 - 3131
Database
ISI
SICI code
0305-1048(1995)23:16<3126:MLDIMO>2.0.ZU;2-1
Abstract
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TO F) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized stran d could be detected. Partial denaturation was found when the duplex pr obes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence anal ysis and DNA diagnostics.