CHROMOSOMAL LOCALIZATION OF MOUSE AND HUMAN GENES ENCODING THE SPLICING FACTORS ASF SF2 (SFRS1) AND SC-35 (SFRS2)/

The mammalian SR-type splicing factors ASF/SF2 and SC-35 play crucial roles in pre-mRNA splicing and have been shown to shift splice site ch oice in vitro. We have mapped the ASF/SF2 gene in mice and humans and the SC-35 gene in mice. Somatic cell hybrid mapping of the human ASF/S F2 gene (SFRS1 locus) reveals that it resides on chromosome 17, and fl uorescence in situ hybridization refines this localization to 17q21.3- q22. Recombinant inbred mapping of the mouse ASF/SF2 gene (Sfrs1 locus ) and the mouse SC-35 gene (Sfrs2 locus) demonstrates that both genes are located in a part of mouse chromosome 11 that is homologous to hum an chromosome 17. Mapping of Sfrs1 using F-1 hybrid backcross mice bet ween the strains C57BL/6 and DDK places Sfrs1 very near the marker D11 Mit38 and indicates that the ASF/SF2 gene is closely linked to the Ovu m mutant locus. (C) 1995 Academic Press, Inc.